PROBLEM: To separate various chemical substances by chromatography.
MATERIALS: 2 plastic cups or containers made from plastic bottles, water (solvent), 4 strips of filter paper, tape, black pen (Bic banana), and other dyes, 2 straighten paper clips. [ With some dyes, you may have to try using Isopropyl alcohol for a solvent, if water has no effect. Do not breath the fumes, cover the container with a piece of plastic or a plastic plate, that you can discard. ]
PROCEDURE AND OBSERVATION:
1. Pour a very small amount of water into a plastic cup.
2. Obtain several strips of filter paper. If no filter paper, try instead using paper towels, one centimeter by fourteen centimeters strips. Make sure that the paper towel used is single layer.
3. About 1 cm from the bottom end of the paper strip; using the black marker, draw a straight line across the strip.
4. If you have food coloring; use a soda top for a container, place a drop of each dye that you may have, mix with a tooth pick. Then using the tooth pick, try to make lines near the ends two strips.
5. Using other black dyes or black markers ( Caution - do not use Indian black ink ) make black lines near the end of the fourth strip.
6. Place a straighten paper clip over each plastic cup. You can only fit two strips in one plastic cup.
7. Fold the fourteen centimeter in such a way that when you hang it on the paper clip, the end with the line makes contact with the water.
8. Be sure, to have the ends of the paper in water.
9. The black lines or markings should not be in the water.
10. The filter paper should hang freely, and should not touch the sides or other strip of paper.
11. After about 5 min. describe what is happening. Continue to observe from time to time for about 20 min. And record your observation.
12. Save the strips of paper, by letting them dry and taping them into the lab book.
QUESTIONS:
1. Define capillary action.
2. Where can capillary action occur.
3. What forces are involved in capillary action ? (3)
4. Would a wick of an alcohol burner make use of capillary action ?
5. List several other places where wicks are used.
6. Observe the strips of paper and describe what changes occurred. ( Try to be complete )
7. Why do you think that these color changes has taken place ?
8. If a liquid contained several soluble substances, could they be separated by chromatography? (Explain)
9. Read II Corinthians 6:17. How would you interpret this verse ?
10. Read I Corinthians 9:24, and interpret this verse.
11. Read Hebrews 12:1, and interpret this verse.
12. Matthew 12:20 A bruised reed shall he not break, and smoking flax shall he not quench, till he send forth judgment unto victory.